CÁC BÀI BÁO KHOA HỌC 07:09:34 Ngày 23/09/2020 GMT+7
NAD-malic enzyme affects nitrogen fixing activity of Bradyrhizobium Japonicum USDA 110 bacteroids in soybean nodules

The NAD+-dependent malic enzyme (DME) has been reported to play a key role supporting nitrogenase activity in bacteroids of Sinorhizobium meliloti. Genetic evidence for a similar role in Bradyrhizobium japonicum USDA110 was obtained by constructing a dme mutant. Soybean plants inoculated with a dme mutant did not show delayed nodulation, but formed small root nodules and exhibited significant nitrogen-deficiency symptoms. Nodule numbers and the acetylene reducting activity per nodule as a dry weight value 14 and 28 days after inoculation with the dme mutant were comparable to those of plants inoculated with wild-type B. japonicum. However, shoot dry weight and acetylene reducting activity per nodule decreased to ca. 30% of the values in plants with wild-type B. japonicum. The sucrose and organic acid (malate, succinate, acetate, a-ketoglutarate and lactate) contents of the nodules were investigated. Amounts of sucrose, malate and a-ketoglutarate increased on inoculation with the dme mutant, suggesting that the decreased DME and nitrogenase activities in the bacteroids resulted in a reduction in the consumption of these respiratory metabolites by the nodules. The data suggest that the DME activity of B. japonicum bacteroids plays a role in nodule metabolism and supports nitrogen fixation.

 Van Dao T., Nomura M., Hamaguchi R., Kato K., Itakura M., Minamisawa K., Sinsuwongwat S., Le H.T.-P., Kaneko T., Tabata S., Tajima S.
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  Từ khóa : Bradyrhizobium japonicum; Bradyrhizobium japonicum USDA 110; Glycine max; Sinorhizobium meliloti