1. Full name: Thai Hanh Dung                                        2. Sex: Female

3. Date of birth: 19/06/1996                                            4. Place of birth: Quang Ninh

5. Admission decision number 3890/QĐ-ĐHKHTN dated on 21/11/2018 by the Rector of University of Science, Vietnam National University, Hanoi

6. Changes in academic process: Extension decision number 151/QĐ-ĐHKHTN dated on 17/01/2023 by the Rector of University of Science, Vietnam National University, Hanoi

7. Official thesis title: Development of novel transformation systems for genetic manipulation in some Aspergillus species

8. Major: Microbiology

9. Code: 9420101.07

10. Supervisors:            Assoc. Prof. Dr. Tran Van Tuan;

Assoc. Prof. Dr. Nguyen Quang Huy

11. Summary of the new findings of the thesis

Newly established Agrobacterium tumefaciens-mediated transformation systems in the two filamentous fungi A. oryzae and A. niger based on the single histidine auxotrophic mutants (DhisB) and dual histidine, uridine/uracil auxotrophic mutants (DhisBDpyrG).

Introducing a new technical solution for deleting multiple genes in a single strain of A. oryzae or A. niger with the pyrG recyclable selection marker.

Providing a new solution to enhance gene deletion efficiency in the two filamentous fungi by using hisB as the first marker for gene deletion and pyrG as the second marker to inhibit ectopic transformants on the transformation plates.

New datasets on phenotypes and biological characteristics of 5 deletion mutants (amyR, laeA, prtT, stuA, veA) and some phytase overexpression strains (phyA) in A. oryzae and A. niger.

12. Further research directions

Exploiting the newly developed ATMT systems and the deletion mutants for recombinant expression of economically valuable enzymes in A. oryzae and A. niger.

Applying the transformation systems to investigate the roles of potential target genes in A. oryzae and A. niger.

13. Thesis-related publications

[1] Hanh-Dung Thai, Bich-Phuong Thi Nguyen, Van-Manh Nguyen, Quang-Huy Nguyen, Van-Tuan Tran (2021), “Development of a new Agrobacterium-mediated transformation system based on a dual auxotrophic approach in the filamentous fungus Aspergillus oryzae”, World Journal of Microbiology and Biotechnology Vol. 37: 92. DOI: 10.1007/s11274-021-03060-z.

[2] Thai Hanh Dung, Tran Van Tuan (2020), “Heterologous phytase expression in the food filamentous fungus Aspergillus oryzae using the added rice husk cultivation model”, Academia Journal of Biology Vol. 42(2), pp. 75-84. DOI: 10.15625/2615-9023/v42n2.14985.

[3] Van-Tuan Tran, Hanh-Dung Thai, Tao Xuan Vu (2022), Chapter 9: “Agrobacterium tumefaciens-Mediated Transformation Systems for Genetic Manipulation in Agriculturally Important Fungi”, Series on Biocatalysis Volume 10: Agricultural Biocatalysis: Biological and Chemical Applications, Jenny Stanford Publishing, ISBN: 978-981-4968-48-5, pp. 285-312. DOI: 10.1201/978100331314.