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1. Full name: Nguyen Van Dung 2. Sex: Male
3. Date of birth: 27/05/1981 4. Place of birth: Bac Ninh
5. Admission decision number: No. 4374/QĐ-KHTN-CTSV date on 03/12/2012 by Rector of VNU University of Science.
6. Changes in academic process:
- Extention according to Decision No. 741/QĐ-ĐHKHTN dated on 31/3/2016 and Decision No. 1034/QĐ-ĐHKHTN date on 25/4/2017, signed by the Rector of VNU University of Science;
- Stopping study according to Decision No. 4995/QĐ- ĐHKHTN dated on 29/12/2017 signed by the Rector of VNU University of Science;
7. Official thesis title: Research compounds with anti-HIV-1 protease activity from plant
8. Major: Biochemistry 9. Code: 62420116
10. Supervisors: Prof.Dr. Phan Tuan Nghia
Assoc.Prof.Dr. Bui Phuong Thuan
11. Summary of the new findings of the thesis
- An assay for inhibitory activity for pepsin (an aspartyl protease similar to HIV-1 protease) by diffusion on agar plates containing hemoglobin substrate was set up. Using this assay, 156 different plant extracts were screened for inhibitory activity for pepsin and 48 different extracts with pepsin inhibitory activity were found, which include: Persea americana (seeds), Thach Chau (leaf), Psidium guajava (leaf), Ephedra distachya (All), Leea rubra (leaf), Diospyros kaki (leaf), Acacia pennata (stem).
- Seven HIV-1 protease inhibitors from plants, including: maslinic acid from Leea rubra, 24-hydroxyusolic acid from Diospyros kaki, 21β-O-[(2E)-6-hydroxyl-2,6-dimethyl-2,7-octadienoyl] pitheduloside G (21 Nonyl-pithethuloside G), iricariside E5, ginsenoside Rg1, rotungenic acid and pitheduloside G from Acacia pennata were purified and obtained. Among these compounds, for the first time maslinic acid was isolated from Leea rubra leaves and a new saponin 21 Nonyl-pithethuloside G from Acacia pennata was found, isolated, structurally studied and characterized. This this also the first report on the anti-HIV-1 protease activity of 24-hydroxyursolic acid, iricariside E5, ginsenoside Rg1, rotungenic acid and pitheduloside G.
- Some properties including inhibition curves, mode of inhibition and cytotoxicity against human embryonic kidney 293T cells of the 7 protease HIV-1 inhibitors were determined.
12. Practical applicability, if any:
- The research results of the study are the basis for the screening, isolation, and characterization of plant-derived HIV-1 protease inhibitors.
- The success of the project will be the foundation for the development of HIV-1 protease inhibitors.
13. Further research directions, if any
- Continuing research on the inhibitory capacity of seven compounds were purified (maslinic acid, 24-hydroxyursolic acid, icariside E5, axit rutongenic, ginsenoside Rg1, pitheduloside G and 21Nonyl-pithethuloside G) and research HIV-1 protease on cellular models and ability to develop into AIDS drugs.
14. Thesis-related publications:
[1] Nguyễn Văn Dũng, Lương Thị Kim Châu, Nguyễn Thị Hồng Loan, Nguyễn Thị Phương, Phương Thiện Thương, Phan Tuấn Nghĩa, Bùi Phương Thuận (2015), “Inhibitory Effect of Plant Extracts on Pepsin and HIV-1 Protease”, VNU Journal of Science, 31(2), pp. 18-27.
[2] Nguyễn Hồng Anh, Nguyễn Thị Hồng Loan, Nguyễn Văn Dũng, Phương Thiện Thương, Bùi Phương Thuận, Phan Tuấn Nghĩa (2015), “A HIV-1 Protease Inhibitor from Psidium guajava L. Leaves”, VNU Journal of Science, 31(4S), pp. 8-15. (PhD student use only the following section screen HIV-1 Protease Inhibitor from Psidium guajava L. Leaves).
[3] Van-Dung Nguyen, Hong-Loan Thi Nguyen¸ Linh-Chi Do, Vu Van Tuan, Phuong Thien Thuong, Tuan-Nghia Phan (2018), “A new saponin with anti-HIV-1 protease activity from Acacia pennata”, Natural Product Communications, 13(4), pp. 411-414.
[4] Nguyễn Văn Dũng, Nguyễn Thị Hồng Loan, Phương Thiện Thương, Bùi Phương Thuận và Phan Tuấn Nghĩa (2018), “24-hydroxyursolic acid from persimmon (Diospyros kaki L.) leaves inhibits HIV-1 protease”, Proceeding of the 3 national scientific conference on biological research and teaching in Viet Nam, Quy Nhon, May 20, 2018, pp. 713– 721
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