We used Fe₃O₄ magnetic nanoparticles (MNPs) which are coated with antiCD4 monoclonal antibody to bind selectively onto membranes of CD4⁺ T cells (hereafter antiCD4-MNPs). The antiCD4-MNPs were prepared through direct covalent interaction between the carboxyl group of the antiCD4 antibody and the amino group of amino-modified MNPs. The antiCD4-MNPs were mixed with human blood cells, followed by bursting the red blood cells with hypotonie buffer; then, the antiCD4-MNPs coated cells were separated by using a magnet. We observed the number of cells bound with magnetite clusters and particles. When fluorescence isothiocyanate labeled antiCD4-MNPs was used to observe the CD4⁺ T cells, the fluorescent intensity was improved by about two times compared to that when cells were labeled with the antiCD4 antibody only. This is a potential method to sort helper CD4⁺ T cells for observation under conventional microscopes.

Khuat N.T., Nguyen V.T.A., Phan T.N., Hoang L.H., Thach C.V., Hai N.H., Chau N.

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